Purification process for production of mannan-binding lectin...

Details Purification process for production of mannan-binding lectin... Purification process for production of mannan-binding lectin...

C - Chemistry, Metallurgy

07

K

C07K 14/47 (2006.01) A61K 38/17 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2334696

The starting material for the present purification process is a supernatant, suspension, milk product, colostrum or crude plasma protein fraction containing MBL. The starting material is subject to few pre-processing steps in order to obtain an MBL containing solution. By this it is possible to perform an affinity chromatography as the first chromatographic process step employing a non-conjugated polysaccharide matrix, from which MBL of a high purity is eluted. Besides constituting the major purification step of the process, the affinity chromatography also serves as a virus-removal step. In the production process of a virus-safe product, a virus-inactivation step is also included. The product of the process of the present invention is ready for use as medicinal product.

Le produit de départ intervenant dans cette technique de purification est un surnageant, une suspension, un produit laitier, un lait colostral ou une fraction de protéine totale du plasma contenant des MBL. On soumet ce produit de départ à quelques opérations de traitements préalables dans le but d'obtenir une solution contenant des MBL. Il est, alors, possible de procéder à une chromatographie d'affinité dont la première phase emploie une matrice polysaccharidique non conjuguée d'où est éluée une MBL très pure. En sus de constituer la principale phase de purification de cette technique, la chromatographie d'affinité sert également de phase d'élimination des virus. Le procédé de production d'un produit exempt de virus comporte également une phase d'inactivation des virus. Le produit obtenu grâce aux techniques de cette invention est prêt à être utilisé comme produit médical.

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