Purification process of tpa

C - Chemistry – Metallurgy – 12 – N

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195/78, 195/39.1

C12N 9/64 (2006.01) C12N 9/72 (2006.01)

Patent

CA 1302929

- 20 - ABSTRACT OF THE DISCLOSURE Tissue plasminogen activator (tPA) species having a molecular weight of about 70,000 daltons is isolated in a purified form from a crude tPA preparation containing various tPA species having different molecular weights by bringing the crude tPA preparation into contact with a cation exchanger in advance and separating the desired tPA species selec- tively from the cation exchanger by means of the salt gradient elution method.

547405

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