Purified alkaline protease concentrate and method of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/54 (2006.01) C11D 3/00 (2006.01) C11D 3/386 (2006.01) C12N 9/56 (2006.01) C12N 15/57 (2006.01) C12S 3/14 (2006.01)

Patent

CA 2086294

A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20 °C and a pH between pH 4.0 and pH 6.5 at constant agitation.

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