Purified dna polymerase from bacillus stearothermophilus

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2186021

Composition and methods for the expression of recombinant DNA polymerase enzymes derived from Bacillus stearothermophilus. The present invention also concerns methods for purifying recombinant Bst DNA polymerase enzymes, compositions containing the purified enzymes in a form suitable for conducting biochemical reactions, and methods for using the purified enzymes.

La présente invention concerne une composition et des procédés d'expression d'enzymes recombinant la polymérase d'ADN de bacillus stéarothermophilus. La présente invention concerne également, des procédés de purification des ADN-polymérases de bacillus stéarothermophilus de recombinaison, des compositions contenant les enzymes purifiées sous une forme convenant à la conduite de réactions biochimiques et des procédés d'utilisation des enzymes purifiées.

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