Purified enzyme and process therefor

Details Purified enzyme and process therefor Purified enzyme and process therefor

C - Chemistry, Metallurgy

12

N

195/123, 195/38

C12N 9/96 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01)

Patent

CA 1301098

X-6961 Abstract Deacetoxycephalosporin C synthetase is pro- vided in purified form via chromatography of crude cell-free extracts over a weak anion exchange resin followed by gel filtration and hydroxylapatite chroma- tography, all carried out in the presence of glycerol, a C1-C3 alkyl monohydric alcohol, e.g., ethanol, a sulfhydryl containing reducing agent, e.g., dithio- threitol, and ascorbate. The purified enzyme which possesses both expandase and hydroxylase activities can be further purified by chromatography over a strong anion exchange resin.

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