Purified enzyme and process therefor

Details Purified enzyme and process therefor Purified enzyme and process therefor

C - Chemistry, Metallurgy

12

N

195/33.1, 530/7.

C12N 9/90 (2006.01) C08K 7/10 (2006.01)

Patent

CA 2018367

X-7790 Abstract Isopenicillin N epimerase is provided in purified form following isolation from cell-free extracts of Streptomyces clavuliqerus. The enzyme of molecular weight of 47,000 (SDS-PAGE) and 50,000 (gel filtration) has been purified to electrophoretic homogeneity, is monomeric, and contains 1 mole of pyridoxal-5'-phosphate per mole of protein.

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