Purified hepatitis c virus envelope proteins for diagnostic...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/51 (2006.01) A61K 39/29 (2006.01) A61K 39/42 (2006.01) C07K 1/113 (2006.01) C07K 14/18 (2006.01) C07K 16/10 (2006.01) G01N 33/576 (2006.01) G01N 33/577 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2172273

The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E2 and/or E1/E2, characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment.

Procédé de purification de protéines de recombinaison d'enveloppe oligomères uniques ou spécifiques du virus de l'hépatite C (HCV) choisies dans le groupe constitué de E1 et/ou E2 et/ou E1/E2, caractérisé par le fait que lors de la lyse des cellules hôtes transformées pour isoler la protéine exprimée par recombinaison, on procède à une étape de clivage ou de réduction de liaison bisulfure à l'aide d'un agent de clivage de liaison bisulfure. La présente invention concerne également une composition isolée grâce au présent procédé, ainsi que l'application diagnostique et thérapeutique desdites compositions. En outre, la présente invention concerne l'utilisation de protéines et de peptides HCV E1 pour le pronostic et la surveillance de l'efficacité clinique et/ou de l'issue clinique du traitement de l'infection par HCV.

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