Purified hepatitis c virus envelope proteins for diagnostic...

A - Human Necessities – 61 – K

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Details

A61K 39/29 (2006.01) C07K 14/18 (2006.01) C12N 15/40 (2006.01) G01N 33/569 (2006.01) G01N 33/576 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2468690

The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E2 and/or E1/E2, characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment.

La présente invention concerne un procédé permettant de purifier des protéines d'enveloppe simple ou d'oligomères spécifiques du VHC (virus de l'hépatite C) recombinant. Ces protéines sont choisies dans le groupe constitué des E1 et/ou E2 et/ou E1/E2. La caractéristique du procédé est qu'au moment de la lyse des cellules transformées, pour isoler la protéine exprimée par recombinaison, on utilise un agent de clivage de la liaison bisulfure de façon à réaliser une réduction ou un clivage de la liaison bisulfure. L'invention concerne également, d'une part une composition isolée par un tel procédé, d'autre part l'application diagnostic et thérapeutique de ces compositions, et enfin l'utilisation des peptides et de la protéine E1 du VHC pour établir un pronostic ou pour surveiller l'efficacité clinique et/ou l'avantage clinique du traitement contre le VHC.

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