Purified herpes simplex virus protease and methods of...

Details Purified herpes simplex virus protease and methods of... Purified herpes simplex virus protease and methods of...

C - Chemistry, Metallurgy

12

N

C12N 9/50 (2006.01)

Patent

CA 2193851

A method of purifying proteases from the Herpes Simplex Virus 1 and 2 is taught. A precursor protease is cloned in a host cell and then separated from the cell culture using cation exchange chromatography. The resulting protease is subjected to autoprocessing conditions and an autolytic cleavage occurs, resulting in mature protease.

L'invention concerne un procédé pour purifier les protéases du virus de l'herpès Simplex 1 et 3. On clone un précurseur de protéase dans une cellule hôte et on le sépare de la culture de cellules en utilisant la chromatographie d'échange cationique. La protéase résultante est soumise à des conditions d'auto-traitement pour produire une scission autolytique, conduisant à la protéase définitive.

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