Purified lh

Details Purified lh Purified lh

C - Chemistry, Metallurgy

07

K

C07K 1/36 (2006.01) C07K 14/59 (2006.01)

Patent

CA 2399100

A process for the purification of recombinant human Luteinizing Hormone (LH) from a sample of crude recombinant LH in the supernatant of CHO cells comprises the combined use of ion-exchange chromatography and reverse phase HPLC. The ion-exchange chromatography and the reverse phase HPLC are performed twice and the final use of a gel permeation column allows the purification from any residual traces of contaminants. The specific bioactivity of the highly purified LH obtained from the process is particularly high, amounting to about 25.000 IU/mg.

L'invention porte sur un procédé de purification de l'hormone lutéinisante (LH) humaine de recombinaison à partir d'un échantillon de LH de recombinaison naturelle dans le surnageant des cellules CHO, ce procédé consistant à utiliser en combinaison la chromatographie d'échange d'ions et HPLC en phase inverse. La chromatographie d'échange d'ions et HPLC en phase inverse sont réalisées deux fois et l'utilisation finale d'une colonne de perméation sur gel permet la purification de toutes traces résiduelles de contaminants. La bioactivité spécifique de LH hautement purifiée obtenue par ce procédé est particulièrement importante, et s'élève à environ 25.000 IU/mg.

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