Purified mannanase from bacillus amyloliquefaciens and...

Details Purified mannanase from bacillus amyloliquefaciens and... Purified mannanase from bacillus amyloliquefaciens and...

C - Chemistry, Metallurgy

12

N

C12N 9/24 (2006.01) C12N 9/42 (2006.01) C12S 3/08 (2006.01) D21C 9/10 (2006.01)

Patent

CA 2230510

According to the present invention, a purified mannanase enzyme obtainable from Bacillus amyloliquefaciens is provided. Preferably, the mannanase of the invention has a molecular weight of about 35,000 when analyzed with the SDS- PAGE method and 20,000 when measured by gel filtration, an isoelectric point (pl) of about 5.2-5.6, a pH optimum of about 4.8-5.2 and a half-life at 80 ~C of about 45 seconds. In another embodiment of the invention, a method is provided for the preparation of a purified mannanase derived from Bacillus amyloliquefaciens.

Cette invention se rapporte à une mannanase purifiée que l'on obtient à partir de Bacillus amyloliquefaciens. De préférence, la mannanase de l'invention a un poids moléculaire d'environ 35000 lorsqu'on procède à une électrophorèse sur gel-SDS et de 20000 lorsqu'on effectue la mesure par filtration sur gel, un point isoélectrique (pl) compris entre 5,2 et 5,6 environ, un pH optimal compris entre 4,8 et 5,2 environ et une demi-vie d'environ 45 secondes à 80 ·C. L'invention se rapporte également à la préparation d'une mannanase purifiée dérivée de Bacillus amyloliquefaciens.

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