Purified nucleic acid encoding a thermostable pyrophosphatase

Details Purified nucleic acid encoding a thermostable pyrophosphatase Purified nucleic acid encoding a thermostable pyrophosphatase

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 9/14 (2006.01) C12N 9/16 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2185362

A DNA that encodes a thermostable pyrophosphatase (PPase) is provided. The DNA is obtained using a DNA probe consisting of SEQ ID NO: 1. Also provided are vectors comprising said DNA and host cells transformed with such a vector. The invention also provides methods for producing thermostable pyrophosphatase, and methods for isolating a target DNA fragment comprising a DNA coding for a thermostable pyrophosphatase from a bacterium of the genus Thermus. The thermostable pyrophosphatase prepared in accordance with the present invention is preferably packed in a kit and can be used in a nucleic acid amplification method.

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