Purified protein a compositions and methods for their...

Details Purified protein a compositions and methods for their... Purified protein a compositions and methods for their...

C - Chemistry, Metallurgy

07

K

530/15.04, 530/3

C07K 1/18 (2006.01) C07K 14/31 (2006.01)

Patent

CA 1339389

Affinity-purified protein A preparations are contacted with a suitable anionic exchange material in order to remove trace contamination. Staphylococcal enterotoxin B (SEB) and other proteinaceous contaminants are removed by passing such affinity-purified protein A preparations through a DEAE-cellulose column and thereafter selectively eluting the protein A to separate the contaminants. Very high purity protein A compositions are thus obtained, typically having a contaminant concentration of about 5 weight % or below, with below about 0.001 weight % SEB.

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