C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/73, 195/1.32
C12P 21/02 (2006.01) A61K 38/20 (2006.01) C07K 1/113 (2006.01) C07K 14/55 (2006.01) C12N 15/26 (2006.01)
Patent
CA 1341083
A process for recovering microbially produced IL-2 in a highly pure form from they cellular material of the microorganism that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
475151
Hanisch Wolfgang H.
Koths Kirston E.
Kunitani Michael
Thomson James W.
Wilson Kenneth
Borden Ladner Gervais Llp
Cetus Corporation
Novartis Vaccines And Diagnostics Inc.
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