Purified recombinant interleukin-2 and process for...

C - Chemistry – Metallurgy – 12 – P

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

195/73, 195/1.32

C12P 21/02 (2006.01) A61K 38/20 (2006.01) C07K 1/113 (2006.01) C07K 14/55 (2006.01) C12N 15/26 (2006.01)

Patent

CA 1341083

A process for recovering microbially produced IL-2 in a highly pure form from they cellular material of the microorganism that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.

475151

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Purified recombinant interleukin-2 and process for... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purified recombinant interleukin-2 and process for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purified recombinant interleukin-2 and process for... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1253526

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.