Purified thermostable nucleic acid polymerase enzyme from...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2092317

2092317 9206202 PCTABS00011 A purified thermostable enzyme is derived from the eubacterium Thermosipho africanus. The enzyme has DNA polymerase activity, reverse transcriptase activity, and optionally 5' $m(8) 3', and/or 3' $m(8) 5' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

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