Purified thermostable nucleic acid polymerase enzyme from...

Details Purified thermostable nucleic acid polymerase enzyme from... Purified thermostable nucleic acid polymerase enzyme from...

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2089495

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima, The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

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