Purified thrombopoietin and method of making it

Details Purified thrombopoietin and method of making it Purified thrombopoietin and method of making it

C - Chemistry, Metallurgy

07

K

C07K 14/52 (2006.01) A61K 38/19 (2006.01) C07K 1/22 (2006.01) C07K 14/715 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2207577

Purified mammalian thrombopoietin proteins and methods of making them are disclosed. The proteins are characterized by a Mr=70,000 ~ 10,000 daltons as determined by SDS-polyacrylamide gel electrophoresis under denaturing conditions and are at least 90 % pure with respect to contaminating proteins. The proteins can be prepared by a method in which thrombopoietin is adsorbed to and eluted from a polypeptide comprising a ligand-binding domain of an MPL receptor, then the eluted thrombopoieting is fractionated by anion exchange chromatography.

L'invention porte sur de la thrombopoïétine purifiée et sur son procédé d'obtention. Ces protéines présentant un M¿r? de 70 000 ~ 10 000 daltons (mesuré par électrophorèse au gel de SDS-polyacrylamide dans des conditions dénaturantes) ont une pureté d'au moins 90 % par rapport aux protéines contaminantes. Elles se préparent à l'aide d'un procédé dans lequel la thrombopoïétine est adsorbée et éluée par un polypeptide comportant le domaine de fixation de ligands d'un récepteur MPL, après quoi la thrombopoïétine éluée est fractionnée par chromatographie par échange d'anions.

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