C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/00 (2006.01) C07K 1/14 (2006.01) C07K 19/00 (2006.01)
Patent
CA 2555137
Described are methods and compositions for producing proteinated granules and isolating desired products (for example, one or more protein, peptide, remediant, cosmeceutical, or pharmaceutical compound species) in transgenic plants engineered to produce the desired product(s). The present invention provides polynucleotide and polypeptide and protein sequences from plants to serve as carriers of recombinant fusion "cargo" proteins (also called "hybrid" or " multimeric" polypeptides), methods for targeting fusion polypeptides to starch granules, methods for tethering fusion polypeptides to the lipid layer associated with starch granule surfaces, and methods for using starch granule associated polypeptides as a carrier protein for the production of recombinant biopharmaceuticals. The desired products are isolated from the transgenic plants by size reduction (also called "milling") and air classification to remove the proteinated granule away from the endogenous host proteome.
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