Pvii phage display

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2697282

The present invention provides an alternative scaffold for peptides displayed on filamentous phages through novel fusion proteins primarily originating from pVII. Libraries of filamentous phages can be created from fusion proteins, and a phage display system comprising a phagemid and a helper phage is a part of the invention.An aspect of the invention is a kit containing a phage display system comprising a phagemid and a helper phage that contains a nucleic acid encoding the fusion protein of the invention.

La présente invention concerne un échafaudage alternatif pour des peptides exprimés sur des phages filamenteux par l'intermédiaire de nouvelles protéines de fusion provenant principalement de pVII. Des banques de phages filamenteux peuvent être créées à partir de protéines de fusion. L'invention concerne également un système d'expression à la surface des phages comprenant un phagemide et un phage auxiliaire. Un aspect de l'invention concerne un kit contenant un système d'expression à la surface des phages comprenant un phagemide et un phage auxiliaire qui contient un acide nucléique codant la protéine de fusion de l'invention.

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