Pyrf gene and its use

Details Pyrf gene and its use Pyrf gene and its use

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/52 (2006.01) C12N 15/80 (2006.01)

Patent

CA 2379796

The invention relates to a pyrF gene and to the utilization thereof as selection marker gene for an expression system for the production of proteins in mushrooms of the genus Trametes, Coriolus or Polyporus. The pyrF gene is characterized in that it comprises DNS sequence SEQ ID NO:1 from position 1133 up to and including position 1877 or DNS sequence SEQ ID NO:2 from position 1 up to and including position 684 or a DNS sequence with a sequence homology greater than 60 % relative to the above-mentioned regions of sequence SEQ ID NO:1 or SEQ ID NO:2.

L'invention concerne un gène pyrF et son utilisation comme gène marqueur de sélection d'un système d'expression pour la production de protéines dans des champignons des genres Trametes, Coriolus ou Polyporus. Le gène pyrF est caractérisé en ce qu'il comprend la séquence DNS SEQ ID NO:1 de la position 1133 à la position 1877 incluse ou la séquence DNS SEQ ID NO:2 de la position 1 à la position 684 incluse ou la séquence DNS avec une homologie de séquence supérieure à 60 % aux zones citées de la séquence DNS SEQ ID NO:1, ou SEQ ID NO:2.

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