Pyruvate carboxylase overexpression for enhanced production...

Details Pyruvate carboxylase overexpression for enhanced production... Pyruvate carboxylase overexpression for enhanced production...

C - Chemistry, Metallurgy

12

N

C12N 9/00 (2006.01) C12N 9/88 (2006.01) C12P 13/00 (2006.01) C12P 13/08 (2006.01)

Patent

CA 2325598

Metabolic engineering is used to increase the carbon flow toward oxaloacetate to enhance production of bulk biochemicals, such as lysine and succinate, in bacterial fermentations. Carbon flow is redirected by genetically engineering the cells to overexpress the enzyme pyruvate carboxylase.

Selon l'invention, on utilise une modification métabolique pour augmenter le flux de carbone vers l'oxaloacétate, afin d'accroître la production de substances biochimiques en vrac, telles que la lysine et le succinate, dans des fermentations bactériennes. On réoriente le flux de carbone en modifiant génétiquement les cellules, afin que celles-ci surexpriment l'enzyme pyruvate carboxylase.

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