Pyruvate producing yeast strain

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/14 (2006.01) C12N 1/16 (2006.01) C12N 1/18 (2006.01)

Patent

CA 2524262

Disclosed herein are glucose tolerant C2 carbon source-independent (GCSI) yeast strains having no detectable amount of pyruvate decarboxylase activity, wherein wild type yeast strains for the glucose tolerant C2 carbon source- independent yeast strains are Crabtree positive. Also disclosed are methods of selecting glucose tolerant C2 carbon source-dependent yeast strains, and methods of producing pyruvic acid or salts thereof using glucose tolerant C2 carbon source-independent yeast strain. Further disclosed herein are GCSI yeast strains having a genome that comprises an exogenous lactate dehydrogenase gene.

L'invention concerne des souches de levure indépendantes d'une source de carbone C¿2? tolérantes au glucose (GCSI), ne présentant aucune quantité détectable d'une activité de pyruvate décarboxylase, dans lesquelles des souches de levure de type sauvage pour les souches de levure indépendantes de source de carbone C¿2? tolérantes au glucose sont positives au test de Crabtree. L'invention concerne également des méthodes de sélection de souches de levure dépendantes de source de carbone C¿2? tolérantes au glucose, et des méthodes de production d'acide ou de sels péruviques de celles-ci, faisant appel à une souche de levure indépendante d'une source de carbone C¿2? tolérantes au glucose. L'invention concerne également des souches de levure GCSI présentant un génome comprenant un gène de lactate déshydrogénase exogène.

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