Quadruplex dna and duplex probe systems

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2422518

A multiplex structure, such as a nucleic acid quadruplex, includes: a first strand containing a first sequence of nucleobases; a second strand containing a second sequence of nucleobases, wherein the second strand is associated with the first strand by Watson-Crick bonding; a third strand containing a third sequence of nucleobases; and a fourth strand containing a fourth sequence of nucleobases, wherein the fourth strand is associated with the second strand and the third strand by Watson-Crick bonding. Formation of the multiplex structure is promoted by monovalent cations (e.g., sodium and potassium), divalent cations, multivalent cations, intercalating agents and/or molecules known to bind within the minor grooves of nucleic acids. The multiplex structure and the process of forming it have diagnostic, therapeutic, prophylactic and nanoengineering applications.

La présente invention concerne une structure multiplex tel qu'un quadruplex d'acide nucléique, comprenant: un premier brin contenant une première séquence de nucléotides; un deuxième brin contenant une deuxième séquence de nucléotides, le deuxième brin étant associé au premier brin par liaison de Watson-Crick; un troisième brin contenant une troisième séquence de nucléotides; un quatrième brin contenant une quatrième séquence de nucléotides, le quatrième brin étant associé au deuxième et au troisième brin par liaison de Watson-Crick. La formation de la structure multiplex est favorisée par des cations monovalents (par ex. sodium et potassium), des cations divalents, des cations plurivalents, des agents intercalants et/ou des molécules connues pour se lier aux sillons mineurs des acides nucléiques. La structure multiplex et son procédé de formation ont des applications diagnostiques, thérapeutiques, prophylactiques et en nanotechnique.

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