Quantification of nucleic acid molecules using multiplex pcr

Details Quantification of nucleic acid molecules using multiplex pcr Quantification of nucleic acid molecules using multiplex pcr

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G06F 19/18 (2011.01) C12P 19/34 (2006.01)

Patent

CA 2705146

Described are novel quantification methods and systems that permit, within the context of multiplex PCR, the quantification of all targets within a single reaction tube. The methods employ quantitation algorithms applied to the amplification profiles of internal calibration controls or standards utilizing a plurality of nucleic acid templates that are amplified within the same reaction tube as the nucleic acid target(s) interrogated.

La présente invention concerne de nouvelles méthodes et de nouveaux systèmes de quantification qui permettent, dans le contexte de la PCR multiplex, de quantifier toutes les cibles dans un seul et unique tube de réaction. Les méthodes font appel à des algorithmes de quantification qui sont appliqués aux profils d'amplification de témoins d'étalonnage internes ou d'étalons au moyen d'une pluralité de matrices d'acides nucléiques qui sont amplifiées dans le même tube de réaction que la ou les cibles d'acides nucléiques interrogées.

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