Quantification of nucleic acids using internal calibration...

Details Quantification of nucleic acids using internal calibration... Quantification of nucleic acids using internal calibration...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2622636

Described herein are approaches to the measurement of gene expression for chosen genes in a biological sample. The methods permit the quantitation of target nucleic acids, e.g., DNAs or RNAs in a nucleic acid sample, both singly and in a multiplex format that permits the determination of levels (e.g., expression levels or copy numbers) for two or more target nucleic acids in a single reaction.

L'invention concerne des approches de la mesure de l'expression génique relative à des gènes choisis dans un échantillon biologique. Les procédés permettent de quantifier des acides nucléiques cibles, dans un format simple mais aussi multiplex permettant de déterminer des niveaux (par exemple des niveaux d'expression ou des nombres de copies) pour deux ou plusieurs acides nucléiques cibles dans une seule réaction.

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