G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 27/327 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2401830
The invention relates to a method for detecting and/or quantifying first biopolymers contained in a liquid involving the following steps: a) providing an electrode with a surface which is made of plastic and which is coated with second biopolymers that have a specific affinity to the first biopolymers to be detected; b) bringing the electrode into contact with the liquid; c) applying a predetermined voltage protocol to the electrode in order to effect a concentration of the first biopolymers on the second biopolymers; d) adding osmium tetroxide and bipyridine to the liquid, and; e) measuring the redox signal coming off the electrode.
L'invention concerne un procédé permettant de détecter et/ou de quantifier de premiers biopolymères contenus dans un liquide, qui comprend les étapes suivantes : a) préparer une électrode comportant une surface en matière plastique et recouverte de seconds biopolymères présentant une affinité spécifique avec les premiers polymères à détecter ; b) mettre en contact ladite électrode avec le liquide ; c) appliquer un protocole de tension prédéfini à l'électrode, de manière à concentrer les premiers biopolymères sur les seconds biopolymères ; d) ajouter du tétroxyde d'osmium et de la bipyridine au liquide ; e) mesurer de signal d'oxydoréduction retombant au niveau de l'électrode.
Grassl Bjorn
Hassmann Jorg
Schulein Jurgen
Fetherstonhaugh & Co.
November Aktiengesellschaft Gesellschaft Fur Molekulare Medizin
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