Quantitation of cellular dna and cell numbers using element...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12Q 1/02 (2006.01) C12Q 1/06 (2006.01) G01N 33/53 (2006.01) G01N 33/567 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2642575

Methods and kits for the quantitation of cellular DNA and cell numbers are provided. Passive element uptake, element-labeled DNA intercalators, and element labeled affinity reagents are used to quantify DNA and cells. The DNA and the cells are analyzed by elemental analysis, including ICP-MS. The methods and kits provide a fast and accurate analysis of cellular DNA and cell numbers.

La présente invention concerne des procédés et des trousses destinés à la quantification d'ADN cellulaire et de cellules. La prise passive d'éléments, des intercalants d'ADN marqués par lesdits éléments, et des réactifs d'affinité marqués par lesdits éléments sont utilisés pour quantifier l'ADN et les cellules. L'ADN et les cellules sont analysés à l'aide d'une analyse élémentaire, incluant l'ICP-MS (spectrométrie de masse à torche plasma). Les procédés et les trousses permettent une analyse rapide et précise de l'ADN cellulaire et du dénombrement des cellules.

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