Quantitation of nucleic acids using growth curves

Details Quantitation of nucleic acids using growth curves Quantitation of nucleic acids using growth curves

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) G01N 21/78 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/536 (2006.01) G01N 33/566 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2482093

Methods, apparatus, and systems, including computer program products, implement techniques for determining an amount of target nucleic acid ("target") in a sample. Signal data is received for a plurality of cycles of an amplification experiment performed on the target and a standard nucleic acid ("standard"). The signal data includes a series of signal values indicating a quantity of standard present during cycles of the standard amplification, and a series of signal values indicating a quantity of target present during cycles of the target amplification. A target growth curve value is defined using the target signal values and a standard growth curve value is defined using the standard signal values. An initial amount of the target is calculated according to a calibration equation using an initial amount of the standard, and the target and standard growth curve values, where the calibration equation is a nonlinear equation.

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