Quantitation of nucleic acids using the polymerase chain...

Details Quantitation of nucleic acids using the polymerase chain... Quantitation of nucleic acids using the polymerase chain...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2064906

The present invention provides a method for determining the amount of a target acid segment in a sample by polymerase chain reaction. The method involves the simultaneous amplification or the target nucleic acid segment and an internal standard nucleic acid segment. The amount of amplified DNA from each segment is determined and compared to standard curves to de- termine the amount of the target nucleic acid segment present in the sample prior to amplification. The method is especially pre- ferred for determining the quantity of a specific mRNA species in a biological sample. Additionally, an internal standard is pro- vided useful for quantitation of multiple mRNA species.

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