Quantitative amplification with a labeled probe and 3' to 5'...

Details Quantitative amplification with a labeled probe and 3' to 5'... Quantitative amplification with a labeled probe and 3' to 5'...

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01)

Patent

CA 2560945

This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3' to 5' exonuclease activity that cleaves the 3' oligonucleotide of the probe.

L'invention concerne des méthodes et des kits permettant de mettre en oeuvre une réaction d'amplification quantitative. La méthode consiste à utiliser une enzyme polymérase et une enzyme à activité exonucléase de 3'-5' coupant l'oligonucléotide 3' de la sonde.

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