G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 24/00 (2006.01) G01N 33/00 (2006.01)
Patent
CA 2502413
The present invention provides for methods of quantitating the amounts of proteins or peptides, including those that are closely related isoforms, using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Measurement of protein concentrations in vivo has been extremely difficult and problematic, and protein concentrations have not been shown to correlate well with mRNA levels, the standard used in the past. The present invention overcomes the deficiencies of prior methodologies by taking advantage of MALDI-TOF-MS technology and applying it to proteins and peptides in a way that allows for accurate, quantitative measurement in vivo of protein or peptide concentrations.
L'invention concerne des procédés de quantification de protéines ou de peptides, y compris ceux constituant des isoformes étroitement apparentées, par spectrométrie de masse à temps de vol par désorption/ionisation laser assistée par matrice (MALDI-TOF-MS). La mesure de concentrations de protéines <i>in vivo</i> est extrêmement difficile et problématique, et les concentrations de protéines ne se prêtent pas bien à des corrélations avec les taux d'ARNm, norme utilisée par le passé. L'invention comble les lacunes des méthodologies antérieures en tirant parti de la technologie MALDI-TOF-MS et en appliquant celle-ci aux protéines et aux peptides, afin d'obtenir des mesures quantitatives précises <i>in vivo</i> de concentrations de protéines ou de peptides.
Duncan Mark W.
Helmke Steve M.
Perryman Benjamin M.
Duncan Mark W.
Helmke Steve M.
Kirby Eades Gale Baker
Perryman Benjamin M.
The Regents Of The University Of Colorado A. Body Corporate
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