Quantitative assays for ras p21 in body fluids

G - Physics – 01 – N

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G01N 33/48 (2006.01) G01N 1/00 (2006.01) G01N 33/00 (2006.01)

Patent

CA 2612693

The present invention is directed to the detection and quantification of total ras p21 in body fluids, particularly serial changes of total ras p21 levels in a subject's body fluids. Further, the invention is directed to detecting and quantitatiing total ras p21 in conjunction with one or more other proteins, such as, oncoproteins, angiogenic factors, tumor markers, inhibitors, growth factor receptors, metastasis proteins, and tumor suppressors. The disclosed methods are diagnostic/prognostic for preneoplastic/neoplastic diseases, and useful to select therapies for patients with preneoplastic/neoplastic diseases. The disclosed methods are further useful to monitor the status of a patient's preneoplastic/neoplastic disease, and/or to monitor how a patient is responding to an anticancer therapy.

La présente invention concerne la détection et la quantification de ras p21 total dans les fluides corporels, en particulier les changements en série de niveaux de ras p21 total dans les fluides corporels d~un sujet. De plus, l~invention porte sur la détection et la quantification de ras p21 total en combinaison avec une ou plusieurs autres protéines, comme les oncoprotéines, les facteurs angiogènes, les marqueurs de tumeur, les inhibiteurs, les récepteurs de facteur de croissance, les protéines de métastase et les suppresseurs de tumeur. Les procédés divulgués sont des diagnostics/pronostics de maladies prénéoplasiques/néoplasiques et sont utiles dans la sélection des thérapies pour les malades prénéoplasiques/néoplasiques. Les procédés divulgués servent également à surveiller le développement d~une maladie prénéoplasique/néoplasique, et/ou à surveiller la réaction du patient à une thérapie anticancéreuse.

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