Quantitative detection of analytes on immunochromatographic...

Details Quantitative detection of analytes on immunochromatographic... Quantitative detection of analytes on immunochromatographic...

G - Physics

01

N

G01N 33/558 (2006.01) G01N 30/90 (2006.01)

Patent

CA 2166913

Disclosed is an improved method for determining the concentration of analyte in a test fluid by immunochroma- tography techniques which involves quantitatively deter- mining the signals from captured analyte/labeled binding partner complex by instrumental means, e.g. reflectance spectrometry. In a preferred embodiment, a reflectance reading is determined for the captured complex and uncom- plexed labeled binding partner which is captured in a separate zone of the immunochromatographic strip and the ratio of these reflectances is used to provide additional quantitation to the assay method.

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