C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2103374
2103374 9221780 PCTABS00017 A method of amplifying and quantitating a specific nucleic acid sequence in a sample using at least one oligonucleotide primer, a supply of nucleotides and optionally a probe complementary to part of the sequence, one of these including a radionuclide. In the presence of a polymerase the reagents form an amplified product based on the specific nucleic acid sequence. The amplified sequence is allowed to bind to a solid material comprising a fluorescer which fluoresces in proximity to the radionuclide and fluorescence is observed as a measure of the specific nucleic acid sequence. The method can be applied to a range of well-known nucleic acid amplification techniques such as the Polymerase Chain Reaction. A variation on the above method allows use of the Ligase Chain Reaction technique.
Blair James Ninian Rowell
Cross Lisa
Downes Malcolm John
Parry David Alun
Robinson Philip Steven
Amersham International Plc
Kirby Eades Gale Baker
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