Quantitative microarray hybridization assays

Details Quantitative microarray hybridization assays Quantitative microarray hybridization assays

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2335447

Methods are provided for quantitative gene expression analysis. In the subject methods, end-labeled target nucleic acid is contacted with an array of probe molecules stably associated with the surface of a solid support under hybridization conditions sufficient to produce a hybridization pattern. The resultant hybridization pattern can be used to obtain a quantitative information about the genetic profile of the end-labeled target nucleic acid sample, as well as the physiological source from which it is derived. As such, the subject methods find use in a variety of applications.

Méthode d'analyse quantitative de l'expression de gènes. Dans les méthodes de l'invention, un acide nucléique cible à marquage terminal est mis au contact d'un arrangement de molécules de sonde associées de manière stable à la surface d'un support solide dans des conditions d'hybridation suffisantes pour produire un modèle d'hybridation. Le modèle d'hybridation résultant peut être utilisé pour obtenir des informations quantitatives sur le profil génétique de l'échantillon d'acide nucléique cible à marquage terminal, ainsi que sur la source physiologique dont il est dérivé. A ce titre, les méthodes de l'invention trouvent une utilisation dans une variété d'applications.

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