Quantitative pcr method of detecting specific plant genus in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2525916

Provided is a method of quantifying a specific plant genus in a food or a food ingredient by a PCR method, comprising: (i) preparing a sample for correction where a sample derived from the specific plant genus to be detected and a standard plant sample are mixed in a predetermined ratio, and extracting genomic DNA from the sample for correction; (ii) preparing a test sample where a known amount of the standard plant sample is added to the food or the food ingredient to be examined, and extracting genomic DNA from the test sample; (iii) practicing a quantitative PCR method using the genomic DNAs and primers; and (iv) conducting correction with a standard value for correction determined for the sample for correction to calculate the amount of the specific plant ingredient contained in the test sample.

L'invention concerne un procédé pour déterminer la quantité d'un végétal de genre déterminé dans les aliments ou les matières premières alimentaires selon la technique PCR. Le procédé consiste à (i) fournir un échantillon destiné à la correction, le rapport de mélangeage du spécimen dérivé à partir du végétal de genre déterminé en tant que cible de détection et un spécimen de plante standard étant identifié d'avance, et extraire les ADN génomiques dudit échantillon; (ii) préparer un échantillon test par l'ajout d'une quantité connue d'un spécimen de végétal standard à un aliment ou à une matière première alimentaire en tant que sujet de test, et extraire les ADN génomiques dudit échantillon; (iii) effectuer une PCR quantitative en utilisant des amorces et ses ADN génomiques; et (iv) tout en effectuant la correction au moyen d'une valeur standard de correction détectée dans l'échantillon pour correction, calculer la quantité de matière première indiquée du végétal d'intérêt, contenue dans l'échantillon test.

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