Quenching reagents and assays for enzyme-mediated luminescence

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/66 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2221522

The present invention relates to single and dual-reporter luminescence assays utilizing general and specific reagents to quench enzyme-mediated reactions. In one embodiment of the invention, a reagent is added to the assay which non- specifically quenches enzyme-mediated luminescent reactions. In another embodiment of the invention, a reagent is added to the assay which simultaneously quenches one enzyme-mediated luminescent reaction while activating another distinct enzyme-mediated luminescent reaction. An assay kit containing specific quench reagents, and the reagents themselves are also disclosed.

La présente invention concerne des dosages de la luminescence par reporter simple ou double faisant intervenir des réactifs généraux ou spécifiques pour éteindre des réactions induites par des enzymes. Dans un mode de réalisation de l'invention, on ajoute au cours du dosage un réactif qui éteint de manière non spécifique les réactions de luminescence induites par des enzymes. Dans un autre mode de réalisation, on ajoute au cours du dosage un réactif qui simultanément éteint une réaction de luminescence induite par des enzymes tout en activant une autre réaction distincte de luminescence induite par des enzymes. L'invention concerne également un nécessaire de dosage contenant des réactifs spécifiques ainsi que ces réactifs mêmes.

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