G - Physics – 01 – N
Patent
G - Physics
01
N
150/11, 167/40
G01N 33/00 (2006.01) G01N 33/94 (2006.01) G21H 5/02 (2006.01)
Patent
CA 1071101
ABSTRACT OF THE DISCLOSURE An improved, simplified radioimmunoassay method for the in vitro determination of cardio- tonic glycosides in unextracted blood serum involves the use of a reagent constituting a buffer solution containing a radioactive hapten for such glycosides. Packaged test kits for use in conveniencely carrying out the radioimmunoassay are also provided. The radioimmunoassay method involves the steps of mixing a sample of blood serum whose cardiotonic glyco- side content is to be determined with a reagent comprising an aqueous buffer solution containing a radioactive hapten for said glycoside, adding to the mixture an antiserum con- taining antibody capable of immunoreactivity with said glycoside and said hapten, incubating the resultant mixture at a temperature and for a sufficient period of time to pro- duce substantial equilibration of the antibody bound hapten and unbound hapten, adding a relatively thin strip of a membrane consisting essentially of an ion-exchange resin to the mixture and maintaining said membrane in contact therewith at a temperature and for a sufficient period of time to separate the unbound hapten from the antibody bound hapten, and determining the relative amounts of antibody bound radioactive hapten and unbound radioactive hapten.
243001
Brown James L.
Lyle Leon R.
LandOfFree
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