C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/9, 150/3.3
C12Q 1/26 (2006.01) C12Q 1/32 (2006.01)
Patent
CA 1221008
ABSTRACT Novel solid compositions are provided for assaying the levels of lower alcohols in body fluids. A carrier is impreg- nated with two or more enzymes, the first of which reduces a reducible agent in the presence of the alcohol. A chromogen is provided which reacts with either the reducer or the oxidized compound in the presence of a second enzyme or reagent, depend- ing on the system, to produce a clinically significant indica- tion of the alcohol present. A quantitative determination may be made by the provision of a competitive inhibitor to the first enzyme to provide a graduated response in colour accord- ing to the concentration of alcohol. Acetaldehyde trapping agents, buffers and enzyme stabilizers are provided as re- quired.
443221
Israel Yedy
Kapur Bhushan M.
Alcoholism And Drug Addiction Research Foundation
Ridout & Maybee Llp
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