C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/32 (2006.01) C12N 9/04 (2006.01) C12N 11/02 (2006.01)
Patent
CA 2083643
ABSTRACT An assay for directly and rapidly determining the alcohol concentration of a lower alcohol in a biological fluid without dilution, comprises a solid carrier. The carrier is impregnated with a solution having the following constituents mixed in effective amounts: a cross-linked stabilized enzyme for oxidizing said alcohol in the presence of a reducible compound to produce a corresponding aldehyde and a reduction product, a chromogen which reacts with the reduction product in the presence of an agent to produce a coloured compound indicative of alcohol presence in said biological fluid, a competitive inhibitor of the enzyme, an agent for converting the chromogen to the coloured compound, and a buffer to maintain the solution at a predetermined acidic pH.
Israel Yedy
Kapur Bhushan
Tu Guang-Chou
Gowling Lafleur Henderson Llp
Israel Yedy
Kapur Bhushan
Tu Guang-Chou
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