Rapid and efficient capture of dna from sample without using...

Details Rapid and efficient capture of dna from sample without using... Rapid and efficient capture of dna from sample without using...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2370122

Nucleic acids can be made available for amplification or other treatment after admixture of a sample with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non- precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. No surfactant or other cell lysing reagents are employed. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.

Les acides nucléiques sont prêts à subir une amplification ou un autre traitement après que l'on a ajouté à un prélèvement des polymères faiblement basiques spécifiques de manière à former un précipité renfermant les acides nucléiques à pH acide. Après enlèvement des substances non précipitées, le pH devient basique, les acides nucléiques se séparant alors du polymère. Le procédé de préparation des échantillons est simple et très rapide et les acides nucléiques libérés peuvent être ultérieurement traités dans des épreuves d'hybridation ou des processus d'amplification. On n'emploie pas de tensioactifs ni de réactifs de lyse cellulaire. Les polymères faiblement basiques sont hydrosolubles et cationiques à un pH acide, mais de charge neutre à un pH basique.

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