Rapid antemortem detection of infectious agents

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/18 (2006.01) G01N 1/40 (2006.01) G01N 33/53 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2756071

Methods for detection of the presence or absence of PrP Sc in a biological sample suspected of having them comprising the steps of concentrating the PrP Sc as may be present in the sample by substantially separating the PrP Sc from the sample matrix; labeling the concentrated PrP Sc with at least one molecular label to produce labeled PrP Sc; and detecting the labeled PrP Sc on an instrument capable of detecting an attomole quantity of labeled PrP Sc, and wherein the duration of time between concentrating the PrP Sc and analyzing the labeled PrP Sc is about 48 hours or less.

La présente invention concerne des procédés permettant de s'assurer de la présence ou de l'absence de protéines PrPSc dans un échantillon biologique suspecté d'en contenir, procédés comprenant les étapes consistant à concentrer les PrPSc susceptibles d'être présentes dans l'échantillon en isolant l'essentiel desdites PrPSc de la matrice de l'échantillon, à marquer lesdites PrPSc concentrées au moyen d'au moins un marqueur moléculaire afin d'obtenir des PrPSc marquées et à détecter lesdites PrPSc marquées au moyen d'un instrument capable de détecter une quantité de l'ordre de l'attomole de PrPSc marquées, la durée s'écoulant entre la concentration des PrPSc et l'analyse des PrPSc marquées étant inférieure ou égale à environ 48 heures.

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