C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2091850
X-8437 Abstract This invention consists of methods for the detection of nucleotide-binding compounds in complex fluid mixtures. The invention consists of fixing a target polynucleotide of known sequence to a non-porous support which comprises or is contained within a container. The challenging sample fluid is then introduced in such a way that compounds present in the fluid bind to the target polynucleotide. This binding blocks the hybridization of a second, complementary polynucleotide to which is attached a label comprising a moiety capable of generating a detectable signal. The invention further provides for indirect means of binding the target polynucleotide to the support and of generating the detectable signal.
Che-Fong Yao Raymond
Meier Timothy Ivan
Eli Lilly And Company
Gowling Lafleur Henderson Llp
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