Rapid assaying method for guanase

Details Rapid assaying method for guanase Rapid assaying method for guanase

C - Chemistry, Metallurgy

12

Q

150/15.1

C12Q 1/34 (2006.01) C12Q 1/28 (2006.01) C12Q 1/62 (2006.01)

Patent

CA 1203154

ABSTRACT The guanase activity in body fluids such as blood serum can be rapidly and accurately assayed by (I) decomposing guanine with the guanase in the specimen to xanthine and ammonia at optimal pH for guanase, suitably pH 7-9, preferably at pH 8, (II) decomposing the xanthine formed by former step I with xanthine oxidase to uric acid and hydrogen peroxide, (III) reacting the reactant solution of the former step II with 3- methyl-2-benzothiazolinonehydrazone, an aniline derivative such as N,N-di-lower-alkylaniline and peroxidase, and finally measuring the optical absorption of the reactant solution of the step III at 570-600 nm. All the steps can be completed within 15 minutes. Therefore, this assay is adoptable for automatic assay of guanase on usual clinically available automatic analysers.

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