C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/48, 195/64.1
C12N 1/00 (2006.01) C02F 3/12 (2006.01) C02F 3/34 (2006.01) C12N 1/30 (2006.01) C12N 9/02 (2006.01) A62D 3/00 (2006.01)
Patent
CA 2003100
Abstract A method is disclosed for degradation of a halogenated hydrocarbon compound such as trichloroethylene (TCE) which utilizes a soluble methane monooxygenase or a bacterium comprising the monooxygenase. Methylosinus trichosporium OB3b is a soluble methane monooxygenase- producing bacterium which when cultivated by continuous culturing comprising exposing the bacterium to a continuous- flow gas mixture of air and methane in a ratio of about 25:1-1:20, respectively. Methylosinus trichosporium OB3b is capable of degrading TCE at rates from about 500-10,000 micromoles per hour per gram cells. The present method is useful to degrade halogenated hydrocarbon compounds such as TCE at initial concentrations up to 10,000 micromoles/l.
Hanson Richard S.
Lipscomb John D.
Gowling Lafleur Henderson Llp
Hanson Richard S.
Lipscomb John D.
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