Rapid determination of glycated hemoglobin

Details Rapid determination of glycated hemoglobin Rapid determination of glycated hemoglobin

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/72 (2006.01) G01N 33/96 (2006.01)

Patent

CA 2102526

2102526 9222818 PCTABS00018 The invention is a rapid, continuous test for glycated hemoglobin using a non-equilibrium affinity binding method. Agarose beads derivatized with 3-aminophenylboronic acid specifically bind glycated hemoglobin. This solid phase is incorporated into a sample processor card, modified to mix and to separate the test solution from the solid phase prior to absorbance readings. Two absorbance readings are made on the test solution, one immediately after mixing the reagent/diluent with the specimen, and one after a significant amount of binding has occurred. A linear correlation between total glycated hemoglobin and hemoglobin A1c permits standardization and reporting of units equivalent to % hemoglobin A1c. Stable glycated hemoglobin solutions for use as standards in the assay, and a method for preparing the standards are also disclosed.

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