Rapid extraction of rna from cells and tissues

Details Rapid extraction of rna from cells and tissues Rapid extraction of rna from cells and tissues

C - Chemistry, Metallurgy

07

H

C07H 21/02 (2006.01) B01D 15/08 (2006.01) C07H 1/06 (2006.01) C07H 1/08 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2463716

A method of extracting RNA from biological systems (cells, cell fragments, organelles, tissues, organs, or organisms) is presented in which a solution containing RNA is contacted with a substrate to which RNA can bind. RNA is withdrawn from the substrate by applying negative pressure. No centrifugation step exceeds thirty seconds. Preferably, the RNA is diluted prior to filtration and one or more wash steps can be used to remove interferents. In one such method, except for DNA shearing and drying steps, centrifugation is not undertaken during the extraction step.

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