Rapid integration site mapping

Details Rapid integration site mapping Rapid integration site mapping

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2465396

High-throughput methods for mapping integration sites resulting from one or more integrations, such as infection by a retrovirus, are disclosed. The disclosed methods require no selection for specific phenotypes such as antibiotic resistance, and thereby may avoid selection bias. Moreover, the linker-based amplification is simple and rapid, and by using a frequently cutting restriction enzyme, the amplicons are small, which significantly decreases possible amplification and cloning biases.

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