Rapid method for detecting micro-organisms and evaluating...

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G01N 33/487 (2006.01) C12Q 1/04 (2006.01) C12Q 1/18 (2006.01) G01N 27/447 (2006.01) G01N 27/60 (2006.01)

Patent

CA 2326320

A method of identifying one or more micro-organisms in a fluid comprising the steps of: (i) taking a sample of the fluid containing a representative sample of any micro-organism(s) present; (ii) optionally culturing the sample if necessary to increase the number of micro-organisms for a pre-determined range; (iii) measuring the zeta potentials of any micro-organisms present; (iv) optionally normalising the measurements taken in step (iii) such that they relate to standard conditions; (v) comparing said measured zeta potentials with a table of the zeta potentials of known micro-organisms to determine which, if any, of the known micro-organisms are present in the fluid.

La présente invention concerne un procédé permettant d'identifier un ou plusieurs micro-organismes dans un fluide. Ce procédé met en oeuvre plusieurs opérations: (i) Prélèvement d'un échantillon de fluide contenant un échantillon de tous les micro-organismes présents. (ii) mise en culture éventuellement de l'échantillon s'il y a besoin d'augmenter le nombre de micro-organismes en vue d'une plage définie. (iii) Mesure des potentiels ? de tous les micro-organismes présents. (iv) Eventuellement rationalisation des mesures tirée de l'opération (iii) de façon à les ramener à des conditions standards. (v) Comparaison des potentiels ? mesurés avec un tableau de potentiels ? de micro-organismes connus afin de déterminer les éventuels micro-organismes connus présents dans le fluide.

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