Rapid method for detection of methicillin resistant...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 1/06 (2006.01)

Patent

CA 2075423

X-8573 Abstract of the Invention The present invention provides a method for the detection of methicillin-resistant staphylococci using the polymerase chain reaction. The reaction detects the presence of the mecA gene, which encodes penicillin binding protein 2A. Also provided is a method for the rapid release of DNA from staphylococci. The two methods can be used in combination for a rapid and sensitive route to the detection of these dangerous pathogens.

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